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Serum sRAGE concentration (ng/mL) in the BALF supernatant was measured using ELISA. 3‐day and 10‐day VEA‐exposed males and females displayed significantly higher serum sRAGE levels compared to control groups. Additionally, 3‐day and 10‐day VEA‐exposed females presented with significantly higher sRAGE levels when compared to their male counterparts. Column height represents mean concentration ± standard error (SE). N = 6/treatment group. BALF, bronchoalveolar lavage fluid; sRAGE, soluble receptor of advanced glycation end products; VEA, vitamin E acetate.

Journal: Physiological Reports

Article Title: Biological sex modulates lung injury severity in adolescent mice exposed to short‐term aerosolized vitamin E acetate

doi: 10.14814/phy2.70691

Figure Lengend Snippet: Serum sRAGE concentration (ng/mL) in the BALF supernatant was measured using ELISA. 3‐day and 10‐day VEA‐exposed males and females displayed significantly higher serum sRAGE levels compared to control groups. Additionally, 3‐day and 10‐day VEA‐exposed females presented with significantly higher sRAGE levels when compared to their male counterparts. Column height represents mean concentration ± standard error (SE). N = 6/treatment group. BALF, bronchoalveolar lavage fluid; sRAGE, soluble receptor of advanced glycation end products; VEA, vitamin E acetate.

Article Snippet: Serum sRAGE levels were quantified using the murine sRAGE Quantikine ELISA kit (Cat. NO. MRG00, R&D Systems, Minneapolis, MN, USA) following the manufacturer's instructions.

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Basal solubilized receptor for advanced glycation end products (sRAGE; A), solubilized Toll-like receptor 4 (sTLR4, C) pre and post 12-week intervention in the control (CON) and aerobic exercise training (AE) groups. Respective absolute changes in basal sRAGE (B) and sTLR4 (C) and percentage changes in sRAGE (E) and sTLR4 (E) were calculated. *P < 0.05 vs Pre, # P < 0.05 vs CON. Statistical analyses performed include mixed model regression analyses with Bonferroni post hoc (panels A & C) and two-tailed t-test (panels B, D, & E).

Journal: medRxiv

Article Title: Aerobic Exercise Training Elevates Circulating sRAGE via Modulation of Sheddase Regulation in Adults with Type 2 Diabetes

doi: 10.1101/2025.09.10.25335517

Figure Lengend Snippet: Basal solubilized receptor for advanced glycation end products (sRAGE; A), solubilized Toll-like receptor 4 (sTLR4, C) pre and post 12-week intervention in the control (CON) and aerobic exercise training (AE) groups. Respective absolute changes in basal sRAGE (B) and sTLR4 (C) and percentage changes in sRAGE (E) and sTLR4 (E) were calculated. *P < 0.05 vs Pre, # P < 0.05 vs CON. Statistical analyses performed include mixed model regression analyses with Bonferroni post hoc (panels A & C) and two-tailed t-test (panels B, D, & E).

Article Snippet: sRAGE was quantified via sRAGE ELISA (DRG000, R&D Systems, Minneapolis, MN) according to manufacturer’s instructions.

Techniques: Control, Two Tailed Test